Unknown: E III A7A

Unknown

Egg diameter in µm	Number of oil globules	Diameter of oil globule in µm	Yolk texture	Perivitelline space	Position of oil globule at hatch	Gut length   at eye- pigment stage	Myomeres
815	1	170	segmented	narrow	bow	60% of NL	24-25

Egg: No image of this egg is currently available. The yellow pigment of the newly hatched larva appears at, or soon after hatching, as none was noted on the developing embryo, which was found to be lightly spotted with black pigment only, with a few spots on the oil globule as well. Incubation is about 30 hours.

Larva: The NH larva is elongate, with a relatively long gut (B, white arrow). The band of yellow along the ventral edge of the notochord behind the yolksac, is almost solid, but does not quite reach the tip (B). The 3-day larva (E) develops prominent black pigment ventrally along the notochord, which is accentuated in the 4-day larva, as the yellow pigment fades, revealing a small patch dorsally near the tail as well (F1). B & C: NH, D: 2 days, E: 3 days, F & F1: 4 days (25°C).

But for the size of the oil globule, the newly hatched larva is very similar to EIIIA3A. The black pigment pattern of the 4-5 day larva, and less elongate shape, differentiate the two. Unfortunately a specimen has not been seen in the past few years, so this larva has not been DNA barcoded.

This egg has been seen off Park Rynie on only 3 occasions, once in January and twice in March. It was also seen once, in November, in the DHM samples.